lördag 11 februari 2012
Restarting, again.
söndag 24 april 2011
Goodbye, Yellow Brick Road!
tisdag 5 april 2011
Safety questions from iGEM Uppsala 2010
- Would any of your project ideas raise safety issues in terms of:
- researcher safety?
During the design process of this project, it was decided that the project idea involved only well-characterized, documented bio-bricks, and non-pathogenic, well-studied bacteria strain. No mutagenic experiments for making new bio-bricks and no pathogenic bacteria strain were ever involved. In the laboratory where the project was carried out, strict safety rules were employed for ensuring the well-being of the project participants. The rules include usage of lab coats, gloves, goggles and other necessary safety equipments. All the lab works were conducted under the supervisions of senior researchers. The project students were not allowed to work alone or without supervisions.
- public safety?
No pathogenic bacteria strains or virulent genes were involved either as precursor material, intermediate or final product. The laboratory where the project carried out is a closed environment. Safety rules were painstakingly followed to prevent the dispersion of, and to ensure the destruction of any lab waste. Autoclave was used to prevent any GMO from leaking out from the lab.
- environmental safety?
No mutations were designed in any of the constructs. The cells which incorporated the artificial plasmid do not secrete toxins as a result. They were destroyed to prevent any form of leakage into the environment. The bacteria strains used in the project possess no selection advantage over their wild type counterparts, other than antibiotics resistance. Although engineering bacteria metabolism through editing antibiotics resistance is a conventional feature.
- Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,
There are no safety issues with our BioBricks. The constructed BioBricks consist of well-characterized parts without any intended mutations. They are only capable of expressing naturally occurring gene activators, repressors and harmless fluorescent proteins. All laboratory procedures were carried out under general microbiology and molecular biology lab regulations.
- Is there a local biosafety group, committee, or review board at your institution?
In
måndag 13 december 2010
Coming up: the recent incident at UU
fredag 10 december 2010
An email to one of Uppsala University's professors
fredag 19 november 2010
今天是个好天气吗?
今天请了一个朋友吃午饭。我事先跟她做了一笔交易。我给她跑腿,帮她一个小忙。她回头要做我安插在某人身边的耳目。从波士顿回来之后我一直都在感情问题上纠结。最后跟这个朋友约好了,她今天代替我问某人一个重要的问题,我作为回报请她吃饭。
其实在她转告我答案之前,我就已经猜到了。只是从她口中听到会帮助我下定决心。按她说,某人对我没有感觉。某人自己认为她已经把态度表明得很明白了。同时她说也怕伤害到我,所以没有说死,没有给我一个 full-blown rejection 的拒绝。我跟亚洲女孩相处的时候,最讨厌的就是这一点。相比之下,白人女孩更加直白。如果她们喜欢你绝对不会让你如此猜测,不喜欢你也会立马说明。喜欢就是喜欢,从第一次见面起几个小时内可以进行到底。不喜欢就是不喜欢,话不投机半句多,上来一句“我已经有男朋友了”,“let us just be friends”,简单明了。男生很快就会知道自己有戏没戏,不会在一个人身上浪费时间。从这点来看,我其实更喜欢欧美人。
当然,什么话都不能说死。我找朋友看的是性格,不是年龄人种肤色身高体重三围。这次喜欢上某人就算是被她成熟气质加天真的组合吸引了。我那位朋友当初听我问起某人,她第一印象就是:“Leo Kinmann的眼光不低啊”。可惜啊可惜。
上面那句可惜是说某人,不是说我。我在波士顿给你买的礼品也要送给别人了。
我这个射手座最阴暗的一面又要亮出来了。人人都说射手男花心,其实不然。射手男是见十个人爱一个,而且两人好起来以后会很专注。很多人们见到的花心射手,其实正是属于寻找模式的。他在与十个人来往之后才能决定谁最适合自己,从而选出那一个进行追逐。那另外九个女生以为自己也被人家看上了,等到射手转移目标才发现,原来醉翁之意不在酒。这下好了,九个女生以为射手欺骗了她们的感情,不专一,花心等种种名号被冠在射手头上。还有一点是射手男事业心很重,这好比当年我在康奈尔忙着找科研实习,仅仅两周就使得Selina被别人先下手为强抢去了。我从那以后就成了现实主义者,不相信什么“总有一个人适合你,你只是还没等到”。因为我知道,即使在两人性格兴趣爱好等等都很般配的前提下,也会因巧合导致阴差阳错而走不到一块去。什么事情都是要人去争取的。只有打了该打的电话,约出去了该请的人,才能有进一步的发展。尤其作为男人根本不能等待天上掉馅饼。何况周围还有那么多竞争对手。
唠叨这么些,我也烦了。末了再说一句话:你要是给我rejection,当我的面直接说。自己心里没那个胆儿面对我还说什么“不想伤害人家”。难道人的心都是纸做的吗?担心会伤害我这个行为本身就是一种对我的侮辱,认为我没那个承受能力。你要么跟我好,要么直接拒掉我。拒了我,跟我做朋友是完全可以的,我接受。但是,不给我任何明了的解释让我等待的所有这类中间行为都是玩弄感情。要把我逼急了,后果会很严重。
lördag 13 november 2010
The first weekend after iGEM
The day I came home, I slept for over 12 hours and woke up at 1pm the day after. I was going to have lunch with an old friend at BMC that day. But since I overslept so much, there was nothing to it. I have had a few very unproductive days recently. In my opinion, I should deserve some of that for working so hard before the Boston trip.
The iGEM Uppsala was not entirely hopeless. We received bronze medal, as I found out once MIT posted the result on their iGEM website. Forgetting to write down the safety questions on wiki wasn't that detrimental to getting the bronze. However, considering most of the teams got gold and there were fewer silver, fewer bronze, and even fewer non-medalists, the bronze is well-deserved but not something I would brandish.
Aside from the academic experience, I met with quite a few Boston-residents that I planned to meet. Allen, Sophia, Mengxiao, and of course my mother's old colleague Jifa. I'm still glad I managed to meet everyone of them during this short visit. After having lunch with Mengxiao in a Harvard dining hall I went shopping. Macy's was a gold mine. Copleys Place and Prudential Center sold mostly expensive stuff for the rich. In the end, I bought a pair of Converse for 45 USD, two shirts and two pairs of pants for 120 USD in total. On top of that I had great food in New England: clam chowder, 1-1/4 lbs lobster times two, great Chinese food, Mexican burritos, and more. In fact food almost got me miss my flight back home. I went away from the boarding gate at Boston Logan airport to buy some takeaway from Panda Express. By the time I was walking back all the way with my fried noodle, kung pao chicken and walnut prawns, my name was called by the staff. They said if I failed to board the plane within 5 minutes, they'd leave without me. I had to rush with my 5 kilo bag and finally made it in time. Although the food was so good it certainly made up for every second of stress I went through.
Back home, Sweden was covered in snow. Compared to Boston's autumn colors, one could imagine how a flight trip can bring you from one season to the next within hours. Quite a few things happened when I was gone. For example, one of my friends got himself involved in quite some drama. But for me, it's time to start working on that damn report and paper. Duh.